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JAVAR Vol 1 Issue 1 (March 2014)                  Published Online: 15 February 2014
J. Adv. Vet. Anim. Res., 1(1): 1-7.     doi: 10.5455/javar.v1i1p1-7
Pathogenicity and immunosuppresive properties of GM-97 strain of infectious bursal disease virus in commercial broiler chicken
Rozina Murmu, Md. Nazrul Islam, Most. Sogra Banu Juli, Md. Abu Sayed Khan, S.M. Harun-ur-Rashid, Ferdaus Mohd. Altaf Hossain, Md. Masudur Rahman
Abstract: The current study was conducted to evaluate the pathogenicity and immunosuppressive effects of GM-97 strain of infectious bursal disease virus in commercial broiler chickens. A total of 500 broiler chickens were vaccinated with the virus through oral route at 10 and 17 days of age (102-103 EID50/dose). Chickens were also vaccinated with Newcastle disease virus (Hitchner B1) orally at 14 and 21 days old. Chickens were euthanized (at 12, 14, 16, 20, 23, 26 days of age) after measuring body weight. Bursa of Fabricius was examined for any gross lesion, weighed and processed for histological investigations.  Bursa to body weight ratio and bursal lesion scoring were made to evaluate pathogenicity of the virus. Blood samples were analyzed for antibody response to ND vaccine virus using HI test. Results showed that the GM-97 strain of IBDV induced mild to moderate depletion of lymphoid cells in the center of bursal follicles and non-significant difference in bursa to body weight ratio amongst vaccinated and unvaccinated chickens. Chickens responded well to ND vaccine by mounting high level of serum NDV specific HI antibody titers. It can be concluded from the present study that GM-97 strain of IBDV has mild pathogenicity but is not immunosuppressive.
J. Adv. Vet. Anim. Res., 1(1): 8-10.   doi: 10.5455/javar.v1i1p8-10
Direct ELISA aided coprological diagnosis of Cryptosporidium parvum infection in diarrheic neonatal calves in Mosul city, Iraq
Israa Al-Robaiee, Maab Al-Farwachi
Abstract: This study was conducted for the detection of Cryptosporidium (C.) parvum infection in neonatal calves. A total of 220 fecal samples (diarrheic 110 and non-diarrheic 110) of neonatal calves were collected from Mosul city, Iraq over a period of 16 months from November 2010 to March 2012. The age of the calves ranged from 1 to 30 days. All the fecal samples were analyzed by capture direct ELISA. The infection was found in 29.0% (n=32/110) of the diarrheic calves. The infection was mostly prevalent (p<0.001) in the calves of three weeks of age. No C. parvum infection could be detected in the non-diarrheic animals.
J. Adv. Vet. Anim. Res., 1(1): 11-15.    doi: 10.5455/javar.v1i1p11-15
Seroprevalence of Mycoplasma gallisepticum infection in backyard and commercial layer chickens in Bhola district, Bangladesh
- Mahfuzul Islam, Jayedul Hassan, Md. Shahidur Rahman Khan
Abstract: This study aimed to determine the seroprevalence of Mycoplasma gallisepticum (MG) infection in the chicken population of Bhola district, Bangladesh, during the period from April 2011 to March 2012. A total of 480 blood samples from chickens were collected from different upazilas (sub-districts) of Bhola district. The sampling considered the types of chicken (backyard and commercial layer), age groups (pullet, adult and old) and seasons (summer and winter). On the basis of the serum plate agglutination test, 55.83% (n=268/480) chickens were found positive for MG. The MG infection was higher (62.5%) in backyard chickens as compared to those being reared in commercial farming systems (53.61%). With respect to age groups, the prevalence was highest in pullets (60.63%) followed by adults (55.63%) and old chickens (51.25%). Moreover, chickens reared in winter showed higher prevalence of MG (60.42%) as compared to those reared in summer (51.25%). In conclusion, MG infection is prevalent in the chicken population of Bhola district, Bangladesh. Appropriate strategies should be taken for successful prevention and control of this disease in Bangladesh.
J. Adv. Vet. Anim. Res., 1(1): 16-20.    doi: 10.5455/javar.v1i1p16-20
Specific antibody response of village chickens to single or combined Newcastle disease and infectious bursal disease vaccines
Abdul-Dahiru El-Yuguda, Saheed Saka Baba, Yaqub Ahmed Geidam
Abstract: This study was conducted to assess the interaction of specific immune responses produced after vaccination using live attenuated Newcastle disease (ND) LaSota and infectious bursal disease (IBD) vaccines in the village chickens of Nigeria. After immunization with the vaccines (individually or in different combinations), specific antibody levels in the chickens were measured using hemagglutination inhibition (HI) and agar gel immunodiffusion (AGID) tests. The cases of administration of ND LaSota alone, ND LaSota followed by IBD vaccine after one week, and simultaneous use of ND LaSota and IBD vaccines were seroconverted against ND virus. Interference of antibody production against ND virus (NDV) or IBD virus (IBDV) was observed when primary vaccination was done by using any one of the two and the remaining one was given after one week. However, simultaneous administration of the vaccines did not interfere with each other in terms of antibody responses. In all the vaccination trials, elicited immunity conferred protection to the chickens challenged with virulent NDV and IBDV. Individual vaccination with ND LaSota followed by IBD vaccines or vice versa giving an interval of more than one week, or simultaneous use of both vaccines are recommended to confer protective antibody levels against NDV and IBDV in village chickens.
J. Adv. Vet. Anim. Res., 1(1): 21-23.    doi: 10.5455/javar.v1i1p21-23
Radio-diagnosis of fecoliths and its non-surgical management in dogs
Arif Khan, Mohsin Ali Gazi, Suhani Bashir, D.M. Dumralia, M.S. Bagate
Abstract: Two cases with a history of cessation of feces for 2 weeks, depression, lethargy and anorexia in two different dogs; a Labrador male and a Doberman female, were presented. On physical examination, abdominal distension along with intense pain was observed. Blood samples were collected from the dogs for hematological and biochemical analyses. The examination revealed an increased percentage of packed cell volume (PCV). By radiological examination, radiopaque material was seen in the large intestinal tract. The condition was suggestive of fecolith. The cases were managed by means of non-surgical management. Through an 11-day long medicinal treatment, the dogs recovered uneventfully.